Conservation of cholera vibrios in complex microcosm containing green microalgae

BACKGROUND: In the summer-autumn period, peak of phytoplankton reproduction and flowering occurs results in deterioration quality river water is noted. At same time, cases gastrointestinal diseases humans are recorded, epidemic complications cholera may occur endemic areas. V. cholerae form biofilm on surface chitinous hydrobionts plastic components can lead to spread cholerae, possibly explaining autochthonous mechanism their existence bodies.
 AIM: This study determines duration Vibrio preservation biotic (chitin) abiotic (plastic) substrates presence green unicellular algae with a change cultivation temperature under experimental conditions.
 MATERIALS AND METHODS: used bacteriological molecular genetic methods achieve its goal.
 RESULTS: The toxigenic non-toxigenic strains O1 El Tor O139 (ctxАВ+tcpА+csh1 ctxАtcpАcsh1+) for six months, including three months at low simulating autumn-winter as part biofilms microcosms, where one component microalgae. We noted an excess concentration serogroups by two orders magnitude samples chitin microalgae substrate. probably stage food chain ecology bodies and, accordingly, be reservoir forms vibrios. Preservation strain viable state decreased temperatures 82C due cold shock gene genome.
 CONCLUSION: Without substrate adhesion, colonization, formation, vibrios incapable long-term persistence temperatures.